Improvement of capillary zone electrophoresis sensitivity with artificial seawater as the background electrolyte utilizing transient isotachophoresis for the determination of nitrite and nitrate ions in seawater

A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphenicol, methylparaben, and propylparaben increased and analysis time reduced when pH of BGE increased from 8.5 to 10.0 and concentration of BGE decreased from 40 mM to 15 mM, but complete separation of chloramphenicol from other matrix components was achieved only with sodium tetraborate concentration at 30 mM or higher and at pH = 9.3 or lower. The most suitable electrophoretic conditions for the intended application were a 30 mM sodium tetraborate solution, pH 9.3 as BGE, working voltage set at 25 kV, and UV detection at 280 nm at the cathodic extremity of the capillary. The final method was validated and proved to be reliable for assay of chloramphenicol, methylparaben, and propylparaben in eye-drops.

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Simultaneous determination of nitrate and nitrite ions in seawater by capillary zone electrophoresis using artificial seawater as the carrier solution

Journal of Chromatography A ◽

10.1016/s0021-9673(99)00214-9 ◽

1999 ◽

Vol 838 (1-2) ◽

pp. 303-311 ◽

Cited By ~ 23

Author(s):

Keiichi Fukushi ◽

Koji Tada ◽

Sahori Takeda ◽

Shin-ichi Wakida ◽

Masataka Yamane ◽

...

Keyword(s):

Simultaneous Determination ◽

Capillary Zone Electrophoresis ◽

Artificial Seawater ◽

Carrier Solution ◽

Zone Electrophoresis ◽

Capillary Zone ◽

Nitrate And Nitrite

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Determination of aristolochic acid by capillary zone electrophoresis

Open Chemistry ◽

10.2478/bf02476197 ◽

2004 ◽

Vol 2 (3) ◽

pp. 417-424 ◽

Cited By ~ 4

Author(s):

František Kvasnička ◽

Rudolf Ševčík ◽

Michal Voldřich ◽

Jana Krátká

Keyword(s):

Detection Limit ◽

Capillary Zone Electrophoresis ◽

Aristolochic Acid ◽

Background Electrolyte ◽

Ease Of Use ◽

Clear Separation ◽

Zone Electrophoresis ◽

Capillary Zone ◽

Running Cost

AbstractA simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5 minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC.

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